Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Tilmicosin (TIM) in the sample such as tissue, liver, honey, eggs and milk. The kit is composed of Microtiter Plate coated with coupled antigens, TIM-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the TIM in the samples will compete with the coupled antigens to combine with anti-TIM antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with TIM content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. TIM residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.5ppb (ng/mL)

>Reactive Mode: 25℃, 30min～15min

>Detection Limits:

Sample

Detection Limits

Animal tissue (higher detection limit)

0.5ppb

Animal tissue (lower detection limit)

10ppb

Liver (chicken liver, pork liver, etc.)

10ppb

Honey

1ppb

Eggs, milk

5ppb

>Cross-reaction Rate:

Tilmicosin………………………………………………100%

Tylosin…………………………….....................................1%

>Sample Recovery Rate:

Sample

Recovery Rate

Animal tissue (higher detection limit)

90±20%

Animal tissue (lower detection limit)

90±20%

Liver (chicken liver, pork liver, etc.)

75±15%

Honey

95±25%

Eggs, milk

95±25%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb

1×1.0mL

High Standard：100ppm(red cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Concentrated Redissolving Solution(2×) (yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1