Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Sulfonamides (SAs) in the sample such as tissue, serum, honey, milk, urine and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, SAs-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the SAs in the samples will compete with the coupled antigens to combine with anti-SAs antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with SAs content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. SAs residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.5ppb (ng/mL)

>Reactive Mode: 25℃, 45min～15min

>Detection Limits:

Sample

Detection Limits

Tissue (higher detection limit)

0.5ppb

Tissue (lower detection limit)

2.5ppb

Serum, urine

2ppb

Honey

0.5ppb

Milk

10ppb

Eggs

1ppb

>Cross-reaction Rate:

Drug name

Cross-reaction Rate

Sensitivity

/ppb

Sulfamethazine(SM2)

100%

0.5

Sulfamonomethoxine(SMM)

670%

0.07

Sulfamerazine(SM1)

313%

0.15

Sulfadiazine(SD/SDZ)

308%

0.15

Sulfadimethoxine(SDM)

175%

0.3

Sulfamethizole(SMT)

165%

0.3

Sulfaquinoxaline(SQX)

42%

1


>Sample Recovery Rate:

Sample

Recovery Rate

Tissue, honey

95±25%

Urine, milk, serum, eggs

85±25%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb

1×1.0mL

High Standard：1ppm(red cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Redissolving Solution(2×) (yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1