Product Name

Tetracyclines (TCs) Rapid Test Kit.


Specification20T/Kit; 50T/Kit

Storage Conditions

² The kit shall be stored at 2 to 30℃ in dry environment.

² Expiry date: 12 months. The date of manufacture is presented in the label of the box.

Intended Application

The test kit is used for the detection of tetracycline-class drugs (Tetracyclines, TCs) in fresh milk, tissues, poultry eggs, and honey.

Principle
The tetracycline-class drugs are broad-spectrum antibiotics, primarily including tetracycline, doxycycline, chlortetracycline, and oxytetracycline, among others.

The kit is developed using the principle of competitive colloidal gold Immunochromatographic Assay (GICA). After the sample solution is added to sample hole, if TCs is present, it will bind with gold labeled antibodies, thereby preventing the labeled antibodies from binding to the TCs conjugates on the nitrocellulose membrane.

If the content of TCs in the sample solution is less than detection limit, it will make the test (“T”) line colored (The color is consistent with the control line or deeper) and the result is negative. If the content is greater than detection limit, no color reaction will be produced (or color is lighter than the control line) and the result is positive.

Technique Data

l Kit Sensitivity: 10ppb (ppb=µg/kg)

l Limit of detection (LOD):

Fresh milk………………………..10ppb

Poultry eggs………………………60ppb

Honey…………………………….50ppb

Tissues:


Sample

Tetracyc-

line(ppb)

Oxytetracy-

cline(ppb)

Chlortetra-

cycline (ppb)

Doxycyc-

line(ppb)

Pork, fish, shrimp

10

10

1000

1000

Beef

20

20

2000

2000

Note: The final detection limit for the sample must be calculated by multiplying the kit sensitivity by the dilution ratio used in sample processing.

Kit Content

Package specification

20T/Kit

50T/Kit

Test device (including a gold-labeled well)

20

50

Extraction solution tube

30mL×2

30mL×4

Redissolving Solution tube

10mL×1

10mL×2

Instruction

1

1