This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Enrofloxacin (ENR) in the sample such as honey, animal tissues (chicken, pork, fish, shrimp), milk, milk powder and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, ENR-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the ENR in the samples will compete with the coupled antigens to combine with anti-ENR antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with ENR content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. ENR residues in sample can be concluded.
l Kit Sensitivity: 0.1ppb (ng/mL)
l Reactive Mode: 25℃, 45min~15min
l Detection Limits:
Sample |
Detection Limits |
Tissue (chicken, pork, fish, shrimp) |
0.3ppb |
Honey |
0.4ppb |
Milk |
3ppb |
Milk powder |
6ppb |
Eggs |
3 ppb |
Serum |
3 ppb |
l Cross-reaction Rate:
Enrofloxacin……………………………………………….100%
Oxolinic acid…………………………………………...…..28%
Levofloxacin……………………………………………….10%
Lomefloxacin………………………………………………..4%
Marbofloxacin…………………………………………….....4%
Sarafloxacin………………………………………………....2%
l Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue, honey, milk, milk powder, eggs, serum, etc. |
85±15% |
Reagent |
Specification |
Microtiter Plate |
8wells× 12strips |
Standard: 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb,8.1ppb |
1×1.0mL |
High Standard:100ppb(red cap) |
1×1.0mL |
Antibody solution (blue cap) |
1×5.5mL |
Enzyme conjugate (red cap) |
1×5.5mL |
Substrate Reagent A (white cap) |
1×6mL |
Substrate Reagent B (black cap) |
1×6mL |
Stop Solution (yellow cap) |
1×6mL |
Concentrated Wash Buffer (20×)(white cap) |
1×40mL |
Concentrated Redissolving Solution(5×) (yellow cap) |
1×50mL |
Instructions |
1 |
Adhesive Membrane |
1 |
Sealed bag |
1 |
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