Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Chloramphenicol (CAP) in the sample such as aquatic tissue, livestock tissue, liver, eggs, honey, milk, feed and water. The kit is composed of Microtiter Plate coated with coupled antigens, CAP-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the CAP in the samples will compete with the coupled antigens to combine with anti-CAP antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with CAP content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. CAP residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.025ppb (ng/mL)

>Reactive Mode: 25℃, 30min～30min～15min

>Detection Limits:

Sample

Detection Limits

Tissue, liver, eggs, honey, milk

0.0125ppb

Water

0.05ppb

Eggs

0.1ppb

Urine, serum, casings, feed, milk powder

0.025ppb

>Cross-reaction Rate:

Chloramphenicol …………………………………………100%

Thiamphenicol, Florfenicol……………………………..＜0.1%


>Sample Recovery Rate:

Sample

Recovery Rate

Tissue, liver

85±20%

Eggs, honey, casings

85±25%

Milk, feed

75±25%

Urine, serum

70±20%

Water

90±20%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb

1×1.0mL

High Standard (red cap)：100ppb

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×11mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Redissolving Solution (2×)(yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1