This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Metabolite (AMOZ) ELISA Kit in the sample such as honey, fish, shrimp, poultry and liver. The kit is composed of Microtiter Plate coated with coupled antigens, AMOZ-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the AMOZ in the samples will compete with the coupled antigens to combine with anti-AMOZ antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AMOZ content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. AMOZ residues in sample can be concluded.
l Kit Sensitivity: 0.05ppb (ng/mL)
l Reactive Mode: 25℃, 45min~15min
l Detection Limits:
Sample |
Detection Limits |
Tissue, liver, eggs |
0.1ppb |
Honey, milk, casings |
0.1ppb |
Milk powder, egg powder, feed |
0.1ppb |
Delicatessen |
0.1ppb |
Tissue(Fish, shrimp and other aquatic products) (Because there is a certain interference) |
0.15ppb
|
l Cross-reaction Rate:
Furaltadone Metabolite (AMOZ)…………………...100%
Furazolidone Metabolite (AOZ)……………………<0.1%
Nitrofurantoin Metabolite (AHD)…………………..<0.1%
Nitrofurazone Metabolite (SEM)…………………...<0.1%
l Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue, liver, eggs |
80±25% |
Honey, milk, casings |
75±15% |
Milk powder, egg powder, feed |
85±25% |
Delicatessen |
75±15% |
Reagent |
Specification |
Microtiter Plate |
8wells× 12strips |
Standard: 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb |
1×1.0mL |
High Standard:100ppb(red cap) |
1×1.0mL |
Derivatization Reagent(black cap) |
1×10mL |
Antibody solution (blue cap) |
1×5.5mL |
Enzyme conjugate (red cap) |
1×5.5mL |
Substrate Reagent A (white cap) |
1×6mL |
Substrate Reagent B (black cap) |
1×6mL |
Stop Solution (yellow cap) |
1×6mL |
Concentrated Wash Buffer (20×)(white cap) |
1×40mL |
Redissolving Solution(2×) (yellow cap) |
1×50mL |
Instructions |
1 |
Adhesive Membrane |
1 |
Sealed bag |
1 |
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