Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Ractopamine (RAC) in the sample such as urine, tissue and feed. The kit is composed of Microtiter Plate coated with coupled antigens, RAC-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the RAC in the samples will compete with the coupled antigens to combine with anti-RAC antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with RAC content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. RAC residues in sample can be concluded.

Technique Data

l Kit Sensitivity: 0.1ppb (ng/mL)

l Reactive Mode: 25℃, 30min～15min

l Detection Limits:

Sample

Detection Limits

Urine

0.3ppb

Tissue

0.4ppb

Feed, pork liver

2ppb

l Cross-reaction Rate:

Ractopamine………………………………………………100%

Dobutamine………………………………………………. <1%

Clenbuterol………………………………………………. <0.1%

Salbutamol………………………………………………..<0.1%


l Sample Recovery Rate:

Sample

Recovery Rate

Urine

95±10%

Tissue

90±15%

feed, Pork liver

80±20%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb, 8.1ppb

1×1.0mL

High Standard：100ppb(red cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Concentrated Redissolving Solution(10×)(yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1