This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Sulfonamides (SAs) in the sample such as tissue, serum, honey, milk, urine and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, SAs-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the SAs in the samples will compete with the coupled antigens to combine with anti-SAs antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with SAs content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. SAs residues in sample can be concluded.
>Kit Sensitivity: 0.5ppb (ng/mL)
>Reactive Mode: 25℃, 45min~15min
>Detection Limits:
Sample |
Detection Limits |
Tissue (higher detection limit) |
0.5ppb |
Tissue (lower detection limit) |
2.5ppb |
Pork liver |
2.5ppb |
Serum, urine, eggs |
1ppb |
Honey |
0.5ppb |
Milk |
10ppb |
>Cross-reaction Rate:
Drug name |
Cross-reaction Rate |
Sensitivity /ppb |
Sulfamethazine(SM2) |
100% |
0.5 |
Sulfamonomethoxine(SMM) |
670% |
0.07 |
Sulfamethoxydiazine(SMD) |
582% |
0.09 |
Sulfadoxine(SDM') |
451% |
0.1% |
Sulfamerazine(SM1) |
313% |
0.15 |
Sulfadiazine(SD/SDZ) |
308% |
0.15 |
Sulfisomidine(SM2') |
241% |
0.2 |
Sulfadimethoxine(SDM) |
175% |
0.3 |
Sulfamethizole(SMT) |
165% |
0.3 |
Sulfachloropyrazine(Esb3) |
67% |
0.8 |
Sulfathiazole(ST) |
58% |
0.9 |
Sulfachlorpyridazine(SCPA) |
58% |
0.9 |
Sulfamethoxypyridazine (SMP) |
57% |
0.9 |
Sulfaquinoxaline(SQX) |
42% |
1 |
Sulfisoxazole(SIZ) |
18% |
3 |
>Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue, honey |
95±25% |
Urine, milk, serum, eggs |
85±25% |
Pork liver |
95±25% |
Reagent |
Specification |
Microtiter Plate |
8wells× 12strips |
Standard: 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb |
1×1.0mL |
High Standard:1ppm(red cap) |
1×1.0mL |
Antibody solution (blue cap) |
1×5.5mL |
Enzyme conjugate (red cap) |
1×5.5mL |
Substrate Reagent A (white cap) |
1×6mL |
Substrate Reagent B (black cap) |
1×6mL |
Stop Solution (yellow cap) |
1×6mL |
Concentrated Wash Buffer (20×)(white cap) |
1×40mL |
Redissolving Solution(2×) (yellow cap) |
1×50mL |
Instructions |
1 |
Adhesive Membrane |
1 |
Sealed bag |
1 |
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