Principle and application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Doxycycline(DOX) in the sample such as tissue, honey and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, DOX-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the DOX in the samples will compete with the coupled antigens to combine with anti-DOX antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with DOX content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. DOX residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.05ppb (ng/mL)

>Reactive Mode: 37℃, 30min～30min～15min

>Detection Limits:

Sample

Detection Limits

Tissue, liver, eggs

2ppb

Honey

2ppb

Urine

0.5ppb

Milk

2ppb

Milk powder

4ppb

>Cross-reaction rate:

Doxycycline …………………………………100%

Tetracyclines…………………………………143%

Chlorotetracycline……………………………..15%

Oxytetracycline………………………………..15%


>Sample Recovery rate:

Sample

Recovery rate

Tissue,

85±20%

Liver, eggs

75±20%

Honey

85±20%

Urine

80±20%

Milk, Milk powder

75±20%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Antibody solution (blue cap)

1×5.5mL

High Standard：1.0ppm (The liquid is volatile and needs to be sealed)

1×1.0mL

Standard: 0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb (black cap; All are empty bottles, ready for use)

Enzyme conjugate (red cap)

1×11mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Concentrated Redissolving Solution(5×) (yellow cap)

1×50mL

Instruction

1

Adhesive Membrane

1

Sealed Bag

1