Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Fluoroquinolones (FQNs) in the sample such as feed. The kit is composed of Microtiter Plate coated with coupled antigens, FQNs-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the FQNs in the samples will compete with the coupled antigens to combine with anti-FQNs antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with FQNs content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. FQNs residues in sample can be concluded.

Technique Data

l Kit Sensitivity: 0.1ppb (ng/mL)

l Reactive Mode: 25℃, 45min～15min

l Detection Limits:

l Cross-reaction Rate:

Enrofloxacin………………………………………………100%

Norfloxacin ……………………………………………….240%

Ciprofloxacin……………………………………………....243%

Lomefloxacin………………………………………………172%

Flumequine………………………………………………….80%

Pefloxacin………………………………………………….210%

Danofloxacin……………………………………………….110%

Sarafloxacin………………………………………………....36%

Difloxacin……………………………………………………84%

Enoxacin……………………………………………………..66%

Ofloxacin…………………………………………………….51%

Oxolinic acid…………………………………………...…..106%

Levofloxacin………………………………………………….3%

Marbofloxacin………………………………………………...4%

l Sample Recovery Rate:

Composition of the Kit