Principle and application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Tetracyclines (TCs) in the sample such as tissue, honey and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, TCs-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the TCs in the samples will compete with the coupled antigens to combine with anti-TCs antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with TCs residue content. Lastly, comparing with the standard curve, TCs residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.05ppb (ng/mL)

>Reactive Mode: 37℃, 30min～30min～15min

>Detection Limits:

Sample

Detection Limits

Tissue, liver, eggs

2ppb

Honey

2ppb

Urine

0.5ppb

Milk

2ppb

Milk powder

4ppb


>Cross-reaction rate:

Tetracyclines…………………………………100%

Oxytetracycline ……………………………...107%

Chlorotetracycline…………………………….16.7%

Doxycycline …………………………………4.2%


>Sample Recovery rate:

Sample

Recovery rate

Tissue, liver, egg

75±20%

Honey

80±20%

Urine

80±15%

Milk, Milk powder

75±15%