This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Tylosin (TYL) in the sample such as poultry tissue, honey. The kit is composed of Microtiter Plate coated with coupled antigens, TYL-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the TYL in the samples will compete with the coupled antigens to combine with anti-TYL antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with TYL content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. TYL residues in sample can be concluded.
>Kit Sensitivity: 0.5ppb (ng/mL)
>Reactive Mode: 25℃, 30min~30min~15min
>Detection Limits:
Sample |
Detection Limits |
Poultry tissue |
1ppb |
Honey |
0.5ppb |
Eggs, milk |
25ppb |
>Cross-reaction Rate:
Tylosin……………………………………………………100%
Erythromycin……………………………………………<0.1%
>Sample Recovery Rate:
Sample |
Recovery Rate |
Tissue |
85±10% |
Eggs, honey, milk |
85±15% |
Reagent |
Specification |
Microtiter Plate |
8wells× 12strips |
Standard: 0ppb, 0.5ppb, 1.5ppb, 4.5ppb, 13.5ppb, 40.5ppb |
1×1.0mL |
Antibody solution (blue cap) |
1×5.5mL |
Enzyme conjugate (red cap) |
1×11mL |
Substrate Reagent A (white cap) |
1×6mL |
Substrate Reagent B (black cap) |
1×6mL |
Stop Solution (yellow cap) |
1×6mL |
Concentrated Wash Buffer (20×)(white cap) |
1×40mL |
Concentrated Redissolving Solution (2×) (yellow cap) |
1×50mL |
Instructions |
1 |
Adhesive Membrane |
1 |
Sealed bag |
1 |
If you would like to receive the latest product manuals and brochures, please feel free to let us know which product's information you require, and we will be happy to provide it to you promptly.
Email: Icey@finderbio.com