Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Sulfadiazine (SD) in the sample such as tissue, serum, honey, milk and urine. The kit is composed of Microtiter Plate coated with coupled antigens, SD-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the SD in the samples will compete with the coupled antigens to combine with anti-SD antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with SD content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. SD residues in sample can be concluded.

Technique Data

l Kit Sensitivity: 0.15ppb (ng/mL)

l Reactive Mode: 25℃, 45min～15min

l Detection Limits:

Sample

Detection Limits

Tissue (higher detection limit)

0.15ppb

Tissue (lower detection limit)

0.75ppb

Serum, urine

0.6ppb

Honey

0.15ppb

Milk

3ppb

Eggs,water

0.3ppb

l Cross-reaction Rate:

Drug name

Cross-reaction Rate

Sulfadiazine(SD/SDZ)

100%


l Sample Recover Rate:

Sample

Recovery Rate

Tissue, honey,water

95±25%

Urine, milk, serum

85±25%

Eggs

90±25%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb, 12.15ppb

1×1.0mL

High Standard：1ppb(red cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Redissolving Solution(2×) (yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1