Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Nitrofurantoin Metabolite (AHD) in the sample such as honey, fish, shrimp, poultry and liver. The kit is composed of Microtiter Plate coated with coupled antigens, AHD-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the AHD in the samples will compete with the coupled antigens to combine with anti-AHD antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AHD content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. AHD residues in sample can be concluded.

Technique Data

l Kit Sensitivity: 0.02ppb (ng/mL)

l Reactive Mode: 25℃, 45min～15min

l Detection Limits:

Sample

Detection Limits

Tissue, liver, eggs

0.04ppb

Honey, milk, casings

0.04ppb

Milk powder, egg powder, feed

0.04ppb

Delicatessen

0.04ppb

Tissue(Fish, shrimp and other aquatic products) (Because there is a certain interference)

0.06ppb

l Cross-reaction Rate:

Nitrofurantoin Metabolite (AHD)……………………100%

Furaltadone Metabolite (AMOZ)…………………...<0.1%

Furazolidone Metabolite (AOZ)……………………<0.1%

Nitrofurazone Metabolite (SEM)…………………...<0.1%

l Sample Recovery Rate:

Sample

Recovery Rate

Tissue, liver, eggs

85±25%

Honey, milk, casings

80±20%

Milk powder, egg powder, feed

85±25%

Delicatessen

80±20%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

1×1.0mL

High Standard：100ppb(red cap)

1×1.0mL

Derivatization Reagent(black cap)

1×10mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Redissolving Solution(2×) (yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1