Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Quinolones (QNs) in the sample such as honey, animal tissues (chicken, pork, fish, shrimp), milk, milk powder and eggs. The kit is composed of Microtiter Plate coated with coupled antigens, QNs-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the QNs in the samples will compete with the coupled antigens to combine with anti-QNs antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with QNs content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. QNs residues in sample can be concluded.

Technique Data

l Kit Sensitivity: 0.1ppb (ng/mL)

l Reactive Mode: 25℃, 45min～15min

l Detection Limits:

Sample

Detection Limits

Tissue (chicken, pork, fish, shrimp)

0.3ppb

Honey

0.4ppb

Milk

3ppb

Milk powder

6ppb

Eggs

3 ppb

Urine

0.5 ppb

Serum

3 ppb


l Cross-reaction Rate:

Enrofloxacin………………………………………………100%

Norfloxacin ……………………………………………….240%

Ciprofloxacin……………………………………………....243%

Lomefloxacin………………………………………………172%

Flumequine………………………………………………….80%

Pefloxacin………………………………………………….210%

Danofloxacin……………………………………………….110%

Sarafloxacin………………………………………………....36%

Difloxacin……………………………………………………84%

Enoxacin……………………………………………………..66%

Ofloxacin……………………………………………………51%

Oxolinic acid…………………………………………...…..106%

Levofloxacin………………………………………………….3%

Marbofloxacin………………………………………………...4%

l Sample Recovery Rate:

Sample

Recovery Rate

Tissue, honey, milk, milk powder, eggs, serum, urine.

85±15%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb,8.1ppb

1×1.0mL

High Standard：100ppb(red cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Concentrated Redissolving Solution(5×)(yellow cap)

1×50mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1