Principle and Application

Diethylstilbestrol is a anabolicsteroids extensively utilized to enhance growth in ruminant species. Nonetheless, given the pronounced carcinogenic potential of Diethylstilbestrol, it has been progressively proscribed or stringently restricted in developed regions including Europe and North America.

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Diethylstilbestrol (DES) in the tissue samples. The kit is composed of Microtiter Plate coated with coupled antigens, DES-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the DES in the samples will compete with the coupled antigens to combine with anti-DES antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with DES content. Lastly, comparing with the standard curve, the obtained concentration is multiplied by the sample dilution ratio. DES residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.05ppb (ng/mL)

>Reactive Mode: 25℃, 30min～30min～15min

>Detection Limits:

>Cross-reaction Rate:

Diethylstilbestrol …………………………………………100%

Dienestrol………………………………………………...38.5%

Hexestrol…………………………………………………..8.5%

 Ethinyl estradiol…………………………………………..<0.1%

 Estriol……………………………………………………..<0.1%

>Sample Recovery Rate:

Composition of the Kit