Principle and application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Aflatoxin M1 (AFM1) in the sample such as liquid milk and milk powder. The kit is composed of Microtiter Plate coated with coupled antigens, AFM1-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the AFM1 in the samples will compete with the coupled antigens to combine with anti-AFM1 antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AFM1 residue content. Lastly, comparing with the standard curve, AFM1 residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.05ppb (ng/mL)

>Reactive Mode: 25℃, ,30min～15min

>Detection Limits:

Sample

Detection Limits

Liquid milk

0.1ppb

Milk powder

0.15ppb

Urine

0.5ppb

>Cross-reaction Rate:

Aflatoxin M1 ………………………………………….100%

>Sample Recovery Rate:

Sample

Recovery Rate

Liquid milk

85±15%

Milk powder

80±15%

Urine

80±15%