Intended Use

Peste des Petits Ruminants (PPR) is an acute, contagious disease in small ruminants caused by the Peste des Petits Ruminants Virus (PPRV). The disease is characterized by erosions in the oral cavity and tongue mucosa, shedding of tears, and nasal discharge. Goats are the primary susceptible species, although the disease can also infect sheep, white-tailed deer, and others, but it is not infectious to cattle.

This kit is designed for the detection of antibodies against PPRV in the serum of sheep and goat. It can be used for evaluating the immunization efficacy of PPRV vaccines.

Principle and Application

This kit comprises a coated Microtiter Plate with recombinant   PPRV antigen, antibody solution, enzyme conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the PPRV in the serum or plasma of sheep and goats. During the experiment, diluted samples and antibody solution are added to the plate. After incubation and subsequent washing to remove unbound components, enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. Further washing steps remove unbound enzyme conjugates. Substrate reagents are then added to the wells. If PPRV antibodies are present in the sample, they will inhibit the binding of the antibody solution to the antigens coated on the plate, preventing color development in subsequent reactions. Conversely, color will develop if no PPRV antibodies are present. The intensity of the color is inversely proportional to the concentration of specific antibodies in the sample. The reaction is terminated by adding the stop solution, turning the product yellow. Absorbance is measured at 450 nm using a microtiter plate reader (microplate reader) to determine the presence or absence of PPRV antibodies in the sample.

Composition of the Kit