Intended Use

Goat/Sheep pox is a zoonotic disease caused by the goat/sheep pox virus (GS-PV), characterized primarily by elevated body temperature and the emergence of pustular lesions on the skin.

This assay is designed to detect antibodies against GS-PV in the serum of sheep and goats. It can be utilized for the evaluation of the immunological efficacy of GS-PV vaccines or as an adjunctive diagnostic tool for the disease.

Principle and Application

This kit comprises a coated Microtiter Plate with GS-PV nucleoproteins (as the antigens), enzyme conjugates, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against GS-PV in the serum of sheep and goats. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains antibodies against GS-PV, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound enzyme conjugates, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of antibodies against GS-PV in the sample.

Composition of the Kit