Intended Use

Brucellosis is a zoonotic infectious disease caused by Brucella bacteria, characterized by inflammation of the reproductive organs and the placenta, leading to miscarriages, infertility, and localized lesions in various tissues.

This assay is designed to detect antibodies against BRU in serum or plasma of sheep and goats. It can be used for the evaluation of the immunological efficacy of the BRU vaccine or as an adjunctive diagnostic tool for the disease.

Principle and Application

This kit comprises a coated Microtiter Plate with BRU antigen, enzyme conjugates, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against BRU in the serum or plasma of sheep and goats. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains BRU antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound enzyme conjugates, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of BRU antibodies  in the sample.

Composition of the Kit