Intended Use

Foot and Mouth Disease (FMD) is an acute, febrile, highly contagious disease of cloven-hoofed animals caused by the Foot and Mouth Disease Virus (FMDV). It is characterized by the formation of vesicles and erosions on the oral mucosa, feet, and mammary skin.

This assay is designed to detect antibodies against Foot and Mouth Disease Virus Type O (FMD-O) in the serum of bovine, sheep and goats. It can be used for the evaluation of the immunological efficacy of the FMD-O vaccine.

Principle and Application

This kit comprises a coated Microtiter Plate with FMD-O antigen, antibody solution, enzyme conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay to detect antibodies against FMD-O in the serum of bovine, sheep and goats. During the experiment, test sample and antibody solution are added to the plate. After incubation and subsequent washing to remove unbound components, enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. Further washing steps remove unbound enzyme conjugates. Substrate reagents are then added to the wells. If FMD-O antibodies are present in the sample, they will inhibit the binding of the antibody solution to the antigens coated on the plates, preventing color development in subsequent reactions. Conversely, color will develop if no FMD-O antibodies are present. The intensity of the color is inversely proportional to the concentration of specific antibodies in the sample. The reaction is terminated by adding the stop solution, turning the product yellow. Absorbance is measured at 450 nm using a microtiter plate reader (microplate reader) to determine the presence or absence of FMD-O antibodies in the sample.

Composition of the Kit