Intended Use

Brucellosis is a zoonotic infectious disease caused by Brucella bacteria, characterized by inflammation of the reproductive organs and the placenta, leading to miscarriages, infertility, and localized lesions in various tissues.

This assay is designed to detect antibodies against BRU in the serum or plasma of bovine, sheep and goats. It can be used for the evaluation of the immunological efficacy of the BRU vaccine or as an adjunctive diagnostic tool for the disease.

Principle and Application

This kit comprises a coated Microtiter Plate with BRU antigen, antibody solution, enzyme conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the BRU in the serum or plasma of bovine, sheep and goats. During the experiment, test sample, enzyme conjugates and antibody solution are added to the plate. Following incubation, if BRU antibodies are present in the sample, they will compete with the antibody solution for binding to the antigens coated on the plate. The enzyme conjugates will form a complex with the antibody solution and the coated antigens on the plate.

Subsequently, the plate is washed to remove any unbound components. Substrate reagents are then added to the wells. If BRU antibodies are present in the sample, they will inhibit the formation of a complex between the enzyme conjugates, the antibody solution, and the coated antigens, thereby preventing any color development in the subsequent reaction. On the other hand, color development will occur if no BRU antibodies are present. The intensity of the color is inversely proportional to the concentration of specific antibodies in the sample.

Finally, a stop solution is added to terminate the reaction, turning the product yellow. The absorbance values in each well are measured at a wavelength of 450nm using a Microtiter  plate reader (microplate reader) to determine the presence or absence of BRU antibodies in the sample..

Composition of the Kit