Intended Use

Foot and Mouth Disease (FMD) is an acute, febrile, highly contagious disease of cloven-hoofed animals caused by the Foot and Mouth Disease Virus (FMDV). It is characterized by the formation of vesicles and erosions on the oral mucosa, feet, and mammary skin.

This assay is designed to detect antibodies against Foot and Mouth Disease Virus NSP (FMD-NSP) in the serum of sheep and goats. It can be used for the screening and adjunctive diagnosis of FMD field virus infection.

Principle and Application

This kit comprises a coated Microtiter Plate with recombinant antigens of FMD-NSP, enzyme conjugates, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against FMD-NSP in the bovine serum. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains antibodies against FMD-NSP, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound enzyme conjugates, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of antibodies  against FMD-NSP in the sample.

Composition of the Kit