Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Zearalenone (ZON/ZEN, also known as F-2 mycotoxin), in the sample such as grain, feed, edible oil, beer, and soy sauce. The kit is composed of Microtiter Plate coated with coupled antigens, ZEN-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the ZEN in the samples will compete with the coupled antigens to combine with anti-ZEN antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AFB1 residue content. Lastly, comparing with the standard curve, ZEN residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.3ppb (ng/mL)

>Reactive Mode: 25℃, 30min～15min

>Detection Limits:

Sample

Detection Limits

Cereals and their food raw materials

6ppb

Feed and feed raw materials

18ppb

Corn husk, wheat bran

18ppb

>Cross-reaction Rate:

Zearalenone ………………………………………………100%

Zearalanone ………………………………………………..13%

Zearalanol ………………………………………………… <1%

>Sample Recovery Rate:

Sample

Recovery Rate

Cereals and their food raw materials

90±15%

Feed and feed raw materials

80±15%

Corn husk, wheat bran

80±15%