Principle and Application

Ochratoxins are secondary metabolites produced by certain species of the genera Aspergillus and Penicillium. Among them, Ochratoxin A is the most widely distributed in nature, the most toxic, and has the greatest impact on humans and animals.

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Ochratoxins (OTA) in the sample such as grains (rice, flour, peanuts, soybeans, and others) and feed. The kit is composed of Microtiter Plate coated with coupled antigens, OTA-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the OTA in the samples will compete with the coupled antigens to combine with anti-OTA antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with OTA residue content. Lastly, comparing with the standard curve, OTA residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 1ppb (ng/mL)

>Reactive Mode: 37℃, 30min～30min～15min

>Detection Limits:

Sample

Detection Limits

Grain

5ppb

Feed

10ppb

>Cross-reaction Rate:

Ochratoxin A ………………………………………………100%

>Sample Recovery Rate:

Sample

Recovery Rate

Grains and compounded feeds

85±15%

Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 1ppb, 3ppb, 9ppb, 27ppb, 81ppb (black cap)

1×1.0mL

High Standard (black cap)：100ppb

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

Enzyme conjugate (red cap)

1×11mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1