Principle and application


This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Aflatoxin B1 (AFB1) in the sample such as grains and feed. The kit is composed of Microtiter Plate coated with coupled antigens, HRP conjugate, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the AFB1 in the samples will compete with the coupled antigens to combine with anti-AFB1 antibodies. After adding HRP conjugate, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AFB1 content. Lastly, by comparing the obtained absorbance values with the standard curve, we can calculate the content of AFB1 toxin in the sample.


Technique Data

> Kit Sensitivity: 0.03ppb (ng/mL)

> Reactive Mode: 25℃, 30min～15min

> Detection Limits:

Grains

0.15ppb

Samples with strong water absorption (such as corn, husk and bran)

0.6ppb

Edible oils such as peanut oil

0.6ppb

Food (such as sauces, cookies, pastries) or condiments

0.6ppb

Beer

0.3ppb

Wine, soy sauce, vinegar

0.15ppb

Tea leaf

0.2ppb

Triticeae crops (wheat and so on)

1.2ppb


>Cross-reaction Rate:

Aflatoxin B1 ………………………………………………100%

> Sample Recovery Rate:

Sample

Recovery Rate

Grains

85±15%

Peanut oil

82±15%

Other edible oils

85±15%

Food (such as sauces, cookies, pastries) or condiments

83±15%

Beer

84±15%

Wine, soy sauce, vinegar

87±15%

Tea leaf, Triticeae crops (wheat and so on)

75±15%



>Composition of the Kit

Reagent

Specification

Microtiter Plate

8wells× 12strips

Standard: 0ppb, 0.03ppb, 0.06ppb, 0.12ppb, 0.24ppb,0.48ppb (black cap)

1.0mL each

High Standard：100ppb(black cap)

1×1.0mL

Antibody solution (blue cap)

1×5.5mL

HRP conjugate (red cap)

1×5.5mL

Substrate Reagent A (white cap)

1×6mL

Substrate Reagent B (black cap)

1×6mL

Stop Solution (yellow cap)

1×6mL

Concentrated Wash Buffer (20×)(white cap)

1×40mL

Instructions

1

Adhesive Membrane

1

Sealed bag

1