Principle and Application

This kit adopts the method of indirect competitive enzyme-linked immunoassay (ELISA) to detect Aflatoxin B1 (AFB1) in the sample such as grains and feed. The kit is composed of Microtiter Plate coated with coupled antigens, AFB1-HRP enzyme conjugates, antibodies, standards and other supporting reagents. During the detection, with adding standards or samples, the AFB1 in the samples will compete with the coupled antigens to combine with anti-AFB1 antibodies. After adding enzyme conjugates, take coloration with TMB substrates. Absorbance value of the samples is a negative correlation with AFB1 residue content. Lastly, comparing with the standard curve, AFB1 residues in sample can be concluded.

Technique Data

>Kit Sensitivity: 0.3ppb (ng/mL)

>Reactive Mode: 25℃, 30min～15min

>Detection Limits:

Sample

Detection Limits

Cereal

3ppb

Oiliness materials

3ppb

Concentrate feed, feed

6ppb

>Cross-reaction Rate:

Aflatoxin B1 ………………………………………………100%

>Sample Recovery Rate:

Sample

Recovery Rate

Cereal

90%±10%

Oiliness materials

85%±10%

Concentrate feed, feed

85%±10%