Intended Use

Newcastle disease, also known as pseudo-poultry plague, is an acute, highly contagious infection in poultry caused by the Newcastle Disease Virus (NDV). It is characterized by respiratory distress, diarrhea, neurological dysfunction, and mucosal hemorrhage.

This assay is designed to detect Newcastle Disease Virus (NDV) antibodies in the serum, plasma, or egg yolk of avian species including chickens, ducks, and geese. It can be employed for the assessment of immunization efficacy and as a supportive diagnostic tool.

Principle and Application

This kit comprises a coated Microtiter Plate with NDV antigen, antibody solution, enzyme conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the NDV in the serum, plasma, or egg yolk of avian species. During the experiment, diluted samples and antibody solution are added to the plate. If the sample contains NDV antibodies, they will compete with the antibody solution to bind with the antigen on the plate, thereby preventing subsequent color development. Conversely, color development occurs in the absence of such antibodies. The intensity of the color is inversely proportional to the specific antibody content in the sample. After adding stop solution to terminate the reaction, the product turns yellow. The absorbance values in each well are measured at a wavelength of 450nm using an microtiter plate reader, allowing determination of the presence of NDV antibodies in the sample.

Composition of the Kit