Intended Use

Avian influenza, also known as highly pathogenic avian influenza (HPAI), fowl plague, or European fowl plague, is an acute and highly lethal contagious disease affecting birds, caused by Influenza A virus. It is characterized by acute septicemic death or asymptomatic carrier status.

This assay is designed to detect Avian Influenza H7 Virus (AIV-H7) antibodies in the serum or plasma of avian species. It can be employed for the assessment of immunization efficacy and as a supportive diagnostic tool.

Principle and Application

This kit comprises a coated Microtiter Plate with AIV-H7 antigen, antibody solution, enzyme conjugate, and other accompanying reagents. It employs the principle of competitive enzyme-linked immunosorbent assay to detect antibodies against the AIV-H7 in the serum or plasma of avian species. During the experiment, diluted samples and antibody solution are added to the plate. If the sample contains AIV-H7 antibodies, they will compete with the antibody solution to bind with the antigen on the plate, thereby preventing subsequent color development. Conversely, color development occurs in the absence of such antibodies. The intensity of the color is inversely proportional to the specific antibody content in the sample. After adding stop solution to terminate the reaction, the product turns yellow. The absorbance values in each well are measured at a wavelength of 450nm using an microtiter plate reader, allowing determination of the presence of AIV-H7 antibodies in the sample.

Composition of the Kit