Intended Use

Infectious Bronchitis (IB) is an acute, highly contagious respiratory disease in chickens caused by the Infectious Bronchitis Virus (IBV). The disease has a global distribution and is one of the most significant epidemics severely impacting the poultry industry. Vaccination is the most effective means of prevention and control of Infectious Bronchitis. The level of post-vaccination antibodies serves as an indicator of the vaccine's efficacy and directly influences the immunized flock's resistance to the virus.

This kit is suitable for: the detection of chicken serum antibodies to determine whether the tested chickens have been infected with Infectious Bronchitis Virus (IBV) or to assess the level of antibodies in immunized chickens; evaluating the risk level of IBV infection in the flock; and understanding the level of maternal antibodies in chicks, to provide a reference for formulating an IBV vaccination program.

Principle and Application

This kit comprises a coated Microtiter Plate with recombinant IBV N protein, enzyme conjugate, and other accompanying reagents. It employs the principle of enzyme-linked immunosorbent assay (ELISA) to detect antibodies against the IBV in the chicken serum or plasma. During the experiment, control serum and test sample are added to the plate. After incubation, if the sample contains IBV antibodies, they will bind to the antigens coated on the microtiter plate. Following washing steps to remove unbound components, the enzyme conjugates are added, which specifically binds to the antigen-antibody complexes on the plate. After washing again to remove unbound enzyme conjugates, substrate reagents are added to the wells and react with the enzyme-labeled complexes, resulting in a blue color. The intensity of the color is directly proportional to the amount of specific antibody present in the sample. The reaction is then terminated by adding a stop solution, turning the solution yellow. The absorbance of each well is measured at a wavelength of 450nm using a microtiter plate reader (microplate reader) to determine the presence of IBV antibodies  in the sample.

Composition of the Kit