Intended Use

Foot and Mouth Disease (FMD) is an acute, febrile, highly contagious disease of cloven-hoofed animals caused by the Foot and Mouth Disease Virus (FMDV). It is characterized by the formation of vesicles and erosions on the oral mucosa, feet, and mammary skin.

This assay is designed to detect antibodies against Foot and Mouth Disease Virus Type A (FMD-A) in the serum of swine, bovine, sheep and goat. It can be employed for the assessment of immunization efficacy.

Principle and Application

This kit comprises a coated Microtiter Plate with FMD-A antibodies, antibody solution, antigen solution, enzyme conjugates, and other accompanying reagents. It employs the principle of blocking enzyme-linked immunosorbent assay (blocking ELISA) to detect antibodies against FMD-A in the serum of swine, bovine, sheep and goat. During the experiment, diluted samples and antigen solutions are added simultaneously to an Microtiter plate for incubation. Antibodies in the sample compete with antibodies on the plate for binding to the antigen, thereby preventing the antigen from binding to the plate. After washing, antibody solution is added for further incubation. Subsequent to another washing step, enzyme conjugates are introduced and allowed to incubate. After another washing to remove any unbound enzyme conjugates, TMB substrates are added to the wells. This reacts with the enzyme-labeled complex to produce a blue-colored product. The intensity of the color is inversely proportional to the concentration of specific antibodies in the sample. The reaction is then terminated by adding a stop solution, turning the product yellow. The absorbance values in each reaction well are measured at 450nm using a Microtiter Plate reader (microplate reader) to determine the presence or absence of FMD-A antibodies in the sample.

Composition of the Kit